Journal: Biotechnology for Biofuels

Article Title: Coupling alkaline pre-extraction with alkaline-oxidative post-treatment of corn stover to enhance enzymatic hydrolysis and fermentability

doi: 10.1186/1754-6834-7-48

Figure Lengend Snippet: ELISA screening of glycans solubilized in pre- and post-treatment liquors using a panel of cell wall glycan-directed monoclonal antibodies (mAbs). These results plot the mAb binding affinity for liquors of (A) alkali pre-extracted corn stover at 0.10 g/g and (B) alkaline hydrogen peroxide (AHP) post-treatment of this pre-extracted corn stover with the mAb categories defined in Additional file .

Article Snippet: To conduct the ELISA screens with a comprehensive collection of cell wall glycan-directed mAbs, each liquor sample was loaded onto the ELISA plates (Corning 384-well clear flat-bottom polystyrene high-bind microplate, product #3700) on an equal carbohydrate basis (15 μL per well from a solution of 20 μg/mL carbohydrate) for conducting ELISA screens as described previously [ , ].

Techniques: Enzyme-linked Immunosorbent Assay, Glycoproteomics, Bioprocessing, Binding Assay

Journal: BMC Plant Biology

Article Title: A DUF-246 family glycosyltransferase-like gene affects male fertility and the biosynthesis of pectic arabinogalactans

doi: 10.1186/s12870-016-0780-x

Figure Lengend Snippet: Characterization of RG-I in PAGR- overexpressing Arabidopsis lines and NbPAGR -silenced N. benthamiana . Size exclusion chromatography using a Superdex 200 column of RG-I from NbPAGR -silenced and control N. benthamiana cell walls ( a ) and RG-I from 35S::PAGR-YFP lines and the wild type ( b ). RG-I from NbPAGR- silenced lines eluted later than the control indicating a decrease in the average molecular weight of RG-I domains. RG-I from PAGR-overexpressing Arabidopsis lines eluted slightly earlier than RG-I from the wild type. c ELISA analysis of size exclusion chromatography-purified RG-I with plant-glycan directed monoclonal antibodies

Article Snippet: Purified RG-I samples were dissolved in water and were coated onto ELISA plates [384 well clear flat bottom polystyrene high bind microplate (product no. 3700), Corning Life Sciences] on an equal weight (gravimetric) per well basis (0.5 μg/well).

Techniques: Size-exclusion Chromatography, Control, Molecular Weight, Enzyme-linked Immunosorbent Assay, Purification, Glycoproteomics, Bioprocessing

Journal: mBio

Article Title: Evidence for the Role of a Second Fc-Binding Receptor in Placental IgG Transfer in Nonhuman Primates

doi: 10.1128/mbio.00341-23

Figure Lengend Snippet: Poor placental IgG MAb transfer is associated with a reduced binding to primate FcγR. (A) Multiplexed SPR sensorgrams depicting association and dissociation of major low-affinity rhesus macaque FcγR allotypic variants (rows) across mammalian (m) and plant (p) antibodies (columns); (B) sensorgrams showing binding of the major high-affinity rhesus macaque FcγR1 variant to mammal- and plant-produced forms of PGT121 defined by BLI; (C) equilibrium binding affinities of antibodies across low-affinity FcγR. K D values that could not be confidently estimated were set as exceeding 20 μM, the highest concentration assessed. (D) Plant- and mammal-derived PGT121 and PGT121-LS were assessed for the ability to induce phagocytosis in human THP-1 cells. (E) Levels of IL-2 (optical density at 450 nm [OD 450 ]) detected in supernatant of rhesus FcγR-expressing reporter cell lines induced by culture on antibody-coated plates.

Article Snippet: At the time that cells were added, 384-well high-binding plates (Corning 3700) were coated with 3 μg/mL unconjugated rat anti-mouse IL-2 (BD Biosciences) overnight at 4°C.

Techniques: Binding Assay, Variant Assay, Produced, Concentration Assay, Derivative Assay, Expressing